Inactivation of the coronavirus that induces severe acute respiratory syndrome, SARS-CoV
Identifieur interne : 005B19 ( Main/Exploration ); précédent : 005B18; suivant : 005B20Inactivation of the coronavirus that induces severe acute respiratory syndrome, SARS-CoV
Auteurs : Miriam E. R. Darnell [États-Unis] ; Kanta Subbarao [États-Unis] ; Stephen M. Feinstone [États-Unis] ; Deborah R. Taylor [États-Unis]Source :
- Journal of virological methods [ 0166-0934 ] ; 2004.
Descripteurs français
- KwdFr :
- Animaux, Antiviraux (pharmacologie), Cellules Vero, Cinétique, Concentration en ions d'hydrogène, Facteurs temps, Formaldéhyde (pharmacologie), Glutaraldéhyde (pharmacologie), Inactivation virale, Rayons ultraviolets, Température élevée, Virus du SRAS (), Virus du SRAS (effets des radiations), Virus du SRAS (physiologie).
- MESH :
- effets des radiations : Virus du SRAS.
- pharmacologie : Antiviraux, Formaldéhyde, Glutaraldéhyde.
- physiologie : Virus du SRAS.
- Pascal (Inist)
English descriptors
- KwdEn :
- Animals, Antiviral Agents (pharmacology), Chlorocebus aethiops, Coronavirus, Formaldehyde (pharmacology), Glutaral (pharmacology), Hot Temperature, Hydrogen-Ion Concentration, Kinetics, Method, Microbiology, SARS Virus (drug effects), SARS Virus (physiology), SARS Virus (radiation effects), Severe acute respiratory syndrome, Time Factors, Tissue culture, Ultraviolet Rays, Vero Cells, Virology, Virus Inactivation.
- MESH :
- chemical , pharmacology : Antiviral Agents, Formaldehyde, Glutaral.
- drug effects : SARS Virus.
- physiology : SARS Virus.
- radiation effects : SARS Virus.
- Animals, Chlorocebus aethiops, Hot Temperature, Hydrogen-Ion Concentration, Kinetics, Time Factors, Ultraviolet Rays, Vero Cells, Virus Inactivation.
Abstract
Severe acute respiratory syndrome (SARS) is a life-threatening disease caused by a novel coronavirus termed SARS-CoV. Due to the severity of this disease, the World Health Organization (WHO) recommends that manipulation of active viral cultures of SARS-CoV be performed in containment laboratories at biosafety level 3 (BSL3). The virus was inactivated by ultraviolet light (UV) at 254 nm, heat treatment of 65 °C or greater, alkaline (pH > 12) or acidic (pH < 3) conditions, formalin and glutaraldehyde treatments. We describe the kinetics of these efficient viral inactivation methods, which will allow research with SARS-CoV containing materials, that are rendered non-infectious, to be conducted at reduced safety levels.
Url:
Affiliations:
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Le document en format XML
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<front><div type="abstract" xml:lang="en">Severe acute respiratory syndrome (SARS) is a life-threatening disease caused by a novel coronavirus termed SARS-CoV. Due to the severity of this disease, the World Health Organization (WHO) recommends that manipulation of active viral cultures of SARS-CoV be performed in containment laboratories at biosafety level 3 (BSL3). The virus was inactivated by ultraviolet light (UV) at 254 nm, heat treatment of 65 °C or greater, alkaline (pH > 12) or acidic (pH < 3) conditions, formalin and glutaraldehyde treatments. We describe the kinetics of these efficient viral inactivation methods, which will allow research with SARS-CoV containing materials, that are rendered non-infectious, to be conducted at reduced safety levels.</div>
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